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02 Clean the stoppers Wipe both vial stoppers with alcohol swabs and let them air dry for 1015 seconds

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These samples were then processed for HPLC/MS analysis, similar to the serum metabolism studies, using a Shimadzu HPLC/MS system

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Another interesting approach emerges by focusing on an important role of glutamine and glutamate in the synthesis of non-essential amino acids (NEAAs) (LeBoeuf et al